Agent 47 (Timothy Olyphant) has been educated to become a professional assassin for hire, whose most powerful weapons are his nerve and a resolute pride in his work. 47 is both the last two digits of the barcode tattooed on the nape of his neck, and his only name.

The hunter becomes the hunted when 47 gets caught up in a political takeover. Both Interpol and the Russian military chase the Hitman across Eastern Europe as he tries to find out who set him up and why they're trying to take him out of the game. But the greatest threat to 47's survival may be the stirrings of his conscience and the unfamiliar emotions aroused in him by a beautiful, damaged girl...